Parabacteroides distasonis DNA lab quality control|BNCC372723 |BNCC

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Parabacteroides distasonis DNA lab quality control

Culture medium	Tryptone: 15.0, soy peptone: 5.0, sodium chloride: 5.0, sterile defibrated sheep blood: 5%, agar: 15.0,pH:7.3±0.2(25 ℃)
Subculture procedure	① Prepare 1-2 pieces of the above-mentioned plates (deoxygenate in anaerobic environment for 24 hours in advance);② Open the safety cabinet, burn the top with alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL sterile water into freeze-drying tube, fully dissolve the bacterial powder, and then put it into the plate at 200 μL/piece, and coat it evenly. ④ Place the plate under the above culture conditions, and the strain can be used.
Growth conditions	37 ℃;24-48h; anaerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 2-4mm, round, irregular edges, opaque, white on the front, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacilli, purity: pure
Sharing mode	Public welfare sharing