Human herpesvirus 5 DNA lab quality control|BNCC363996 |BNCC

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Human herpesvirus 5 DNA lab quality control

Culture medium	90%EMEM+10%FBS
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 37 ℃; Culture time 2-7 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	This strain was isolated from the urine of a male patient with congenital cytomegalovirus inclusion disease.
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of human herpes virus 5, and can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing