Human herpesvirus 5 DNA lab quality control|BNCC363558 |BNCC

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Human herpesvirus 5 DNA lab quality control

Culture medium	EMEM Complete Medium: 90% EMEM + 10% FBS
Subculture procedure	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl.
Growth conditions	The culture temperature is 37 ℃; The culture time is 2-7 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	The strain was isolated from the urine of a male patient with congenital cytomegalovirus inclusion disease.
application	The essential control product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It is a human herpes virus 5 inactivated suspension. It can be used for method establishment, work standard assignment, method confirmation and evaluation, and product quality control through digital PCR.
Sharing mode	Public welfare sharing