Shigella flexneri nucleic acid detection standard|BNCC362377 |BNCC

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Shigella flexneri nucleic acid detection standard

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	When this standard is used, it is moved to room temperature (20~25 ℃) for re-melting, oscillates evenly, centrifuges briefly, and is temporarily stored for use in a low temperature environment on ice to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
application	It is suitable for the assessment and verification of laboratory nucleic acid detection capabilities (sensitivity test and specificity test); it can carry out indoor quality control on the relevant laboratories of Shigella flexneri nucleic acid detection, and effectively monitor the status of the instrument, personnel operation, and kit Effectiveness, etc., to ensure the accuracy and reliability of the test results, but not used for reagent evaluation and negative-positive result judgment.
Sharing mode	Public welfare sharing