Bacillus subtilis quantitative strain|BNCC365155 |BNCC

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Quantitative strains of bacillus subtilis

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃, aerobic
Growth characteristics	Gram-positive, rod-shaped, rarely in chains, evenly stained; flagellum lateral, motile; endospores 0.8 × 1.5-1.8 μm, weakly colored on the surface of free spores; colonies round or irregular on the culture medium of foreign vegetables; surface color dark, opaque, can wrinkle, can become cream or brown; the shape of colonies varies greatly with the composition of the culture medium; strictly well-fed or facultative anaerobic.
Storage conditions	-20 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, irregular edges, opaque, gray-white front, flat surface, smooth surface, bright surface, dry texture, difficult to pick/cut, G (blue-purple), bacilli, purity: pure
Sharing mode	Public welfare sharing