New coronavirus ribonucleic acid RNA quality control sample (High value)|BNCC359802 |BNCC

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New coronavirus ribonucleic acid RNA quality control sample (High value)

Subculture procedure	1. After the sample is remelted, it should be temporarily stored in a low temperature environment on ice to avoid repeated freezing and thawing. DNA/RNase-free centrifuge tubes and tips should be used during the experiment. 2. When using this standard substance for ORF1ab gene measurement, please carefully verify the coordinates of the PCR primer sequence to be used in the genome of the novel coronavirus. The primer sequence cannot exceed the range of genomic coordinates described in the "Sample Preparation" section.
Storage conditions	-80 ℃, cold storage away from light; Dry ice transportation
Safety level	1
application	Essential control products are suitable for quality control and process control of internal testing items in relevant laboratories, and are not suitable for diagnosis and fixed value of clinical cases.
Sharing mode	Public welfare sharing

New coronavirus ribonucleic acid RNA quality control sample certificate

1. Product information

Sample name: new coronavirus ribonucleic acid RNA quality control sample (high value)

Sample number: 359802

Sample batch: 220602

2. Product features

Description	Essential controls are packaged in 1.5mL cryovials, each 0.5mL, with a minimum sampling volume of 2μL
Traceability	N gene (full length), E gene (full length) and open reading frame 1ab (ORF1ab) gene fragments of important characteristic genes of novel coronavirus (genome coordinates: 13201-15600, GenBank No. NC_045512).
Parameters	N gene	RF1ab gene	OE gene
Concentration value (copies/mL)	10⁵~10⁶	10⁵~10⁶	10⁵~10⁶
Reference value (copies/mL)	5.1×10⁵	7.4×10⁵	4.8×10⁵
Uniformity and stability	Random sampling, the uniformity of the results is good; long-term stability test, the stability is qualified
Applicable	Essential controls are suitable for quality control and process control of relevant laboratory internal testing items, but are not suitable for diagnosis and determination of clinical cases.
*Note: If you have any questions about quality control samples, please contact our center (BNCC) for help before use.

3. Instructions

1. After the samples are thawed, they should be temporarily stored on ice at a low temperature, and avoid repaeated freezing and thawing. DNA/RNase-free centrifuge tubes and tips should be used during the experiment.

2. When using this standard material for ORF1ab gene measurement, please carefully verify the coordinates of the PCR primer sequences to be used in the novel coronavirus genome. Primer sequences cannot exceed the range of genomic coordinates described in the "Sample Preparation" section.

4. Precautions

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet, and try to avoid repeated freezing and thawing.

3. Pay attention to biosafety protection when using essential control products. It should be treated as a potentially biologically infectious sample, and the operation and handling must comply with relevant regulatory requirements: the Ministry of Health's 《General Guidelines for Biosafety of Pathogenic Microbiology Laboratories》 and 《Medical Waste Management Regulations》.

5. Preservation and transportation

Long-term storage: -80 ℃, valid for 1 year; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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