BeNa Culture Collection
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Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. |
| Subculture procedure | (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions. |
| Growth conditions | 45°C;3-5 days; Aerobic |
| Storage conditions | 2-8 ℃ |
| Separation substrate | wheat straw compost |
| Sharing mode | Public welfare sharing |
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