Lentinus edodes|BNCC360535 |BNCC

BeNa Culture Collection

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Lentinus edodes-BNCC
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  • Lentinus edodes-BNCC

Lentinus edodes

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  • number：BNCC360535
Standard strain Quantitative strain DNA extraction
Package：
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Related Products
Lentinus edodes
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 comprehensive PDA plates; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into pieces with aseptic inoculation hoe and inoculation shovel (see attached page),0.5 × 0.5cm2 small square pieces; (4) flat the small square block to the center of the plate and on the agar surface; (5) the plate is being cultured under the above culture conditions.
Growth conditions 25-28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
application Eat
Sharing mode Public welfare sharing
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