BeNa Culture Collection
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Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min. |
| Subculture procedure | (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow. |
| Growth conditions | 28 ℃;24-48h; Aerobic; |
| Storage conditions | 2-8 ℃ |
| morphology | Ascomycetes yeast. |
| application | Vietnamese feed yeast (starch, raw rice bran available); chicken manure deodorization |
| Sharing mode | Public welfare sharing |
Pichia burtonii(Pichia burtonii)
BNCC140336