Shigella nucleic acid detection kit (fluorescent PCR method)|BNCC361199 |BNCC

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Shigella nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	Sample treatment: sample treatment shall be carried out in accordance with GB/T 4789.5-Detection of Shigella in 2003 Foods; Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL (when diluted bacteria solution is used as template, the addition amount is 10 μL); Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 52 ℃ 30s 40cycle (when the diluted bacteria solution is used as the template for the first step reaction for 3min); Fluorescence channel: FAM;
Storage conditions	-20 ℃; protected from light
Safety level	0
application	It is suitable for the auxiliary diagnosis of Shigella infection in water samples, stool, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing