BeNa Culture Collection
Subculture procedure | 1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: self-prepared or pre-extracted test sample template DNA; 3. Reagent preparation: reaction system (25μL), take 15μL of reaction solution, add 2μL of template and 8μL of DEPC water, mix well, and centrifuge briefly; 4. Reaction conditions: 95 ℃ ,3min ,1cycle;95 ℃,15s ;60 ℃ ,30s ,40cycle. 5. Fluorescence channel: FAM; |
Storage conditions | -20 ℃; protected from light |
Safety level | 1 |
application | Suitable for identification and detection of donkey-derived ingredients |
Sharing mode | Public welfare sharing |