BeNa Culture Collection
Culture medium | Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 28 ℃;5 days; aerobic |
Storage conditions | -80 ℃ |
Safety level | 1 |
morphology | Spreading growth, producing black spores, irregular edges, opaque, black front, rough surface, dry texture |
Separation substrate | Blueberry, North Carolina |
application | Quality Requirements for GB 4789.28 Media and Reagents |
Sharing mode | Public welfare sharing |