Aspergillus brasilia quantitative strain|BNCC364354 |BNCC

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Quantitative strains of aspergillus brasiliensis Varga et al.

Culture medium	Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	28 ℃;7 days; aerobic
Storage conditions	-20 ℃
Safety level	2
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium. The hyphae of the plate layer is white in the early stage and gradually turns black, and produces black spores in the later stage, and the back of the medium is brown and black.
application	Quality Requirements for GB 4789.28 Media and Reagents
Sharing mode	Public welfare sharing