Mycobacterium phlei quantitative strain|BNCC366393 |BNCC

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Quantitative strains of mycobacterium phlei

Culture medium	Tryptone: 15.0, soy peptone: 5.0, sodium chloride: 5.0, agar: 15.0,pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;48-72h; aerobic;
Growth characteristics	The strain was cultured on nutrient meat juice agar medium at 37 ℃ for 3 days, and the thallus was rod-shaped, 0.61 μm × 1.22~2.13 μm, gram-negative, and did not produce spores.
Storage conditions	-20 ℃
Safety level	2
morphology	Size: 1-2mm shape: round edge: irregular transparency: opaque color: yellow uplift: flat surface: rough wrinkled texture: dry and easy to pick up, G + (blue purple), bacilli
Separation substrate	starter culture
Sharing mode	Public welfare sharing