Nine kinds of bacterial diarrhea pathogen nucleic acid detection kit (multiplex fluorescent PCR method)|BNCC376040 |BNCC

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Nine bacterial diarrhea pathogen nucleic acid testing kits (Fluorescent PCR method)

Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 40s, and the second to third steps are cycled 40 times;
Storage conditions	-20 ℃; protected from light
Safety level	1
application	A rapid detection of Vibrio parahaemolyticus, Listeria monocytogenes, Bacillus cereus, cholera, Staphylococcus aureus, Escherichia coli O157:H7, Campylobacter jejuni, Salmonella, Shigella nucleic acid detection
Sharing mode	Public welfare sharing