BeNa Culture Collection
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| Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Growth conditions | 37 ℃, aerobic, 18-24h |
| Storage conditions | -80 ℃; ship in ice bag |
| Separation substrate | Blood culture |
| Sharing mode | Public welfare sharing |
Genomic DNA
Product content: 5μg ;
Purity: (A260/A280):1.7-2.0
Product format: Freeze-dried powder
Storage conditions: 2~8 ℃
Validity period: 6 months
Biosafety level: 1
Purpose: Molecular research
Notes: If you find any abnormal conditions such as damage on the day of receipt, please contact customer service within 24 hours; after opening, it should be used up once and cannot be stored. If it is not opened temporarily, it can be stored at -20°C. Please operate in strict accordance with this instruction, otherwise it will cause abnormality, deactivation, etc., and no reissue service will be provided.
Steps:
1. Prepare sterile ddH2O;
2. centrifuge the sample tube 12000r /min for 1min, and add the corresponding volume of sterile ddH2O as required;
3. It can be used normally after a short period of centrifugation after oscillation;
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