BeNa Culture Collection
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![pLV[Exp]-EGFP-CMV>{HPIV4B}-BNCC](https://beinabncc.oss-accelerate.aliyuncs.com/News/5d2d278691620d2f2ef113124b587296.jpg "DH5α (containing pLV3-CMV-HPIV4B) E. coli")
| Growth conditions | 37°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare one vial of sterile water and two 90mm resistant plates;② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5 mL sterile water into lyophilized vial. After complete dissolution, spread solution onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment; |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
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