DH5α (containing pET22b-EBFP plasmid) E. coli|365215 |BNCC

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Escherichia coli DH5α（pET22b-EBFP）

Subculture procedure	① Prepare 1 vial of sterile water and 2 x 90mm resistant plates;② Disinfect the ampoule surface, open it in a biosafety cabinet, cauterize the top with an alcohol lamp, then immediately add sterile water to rupture it. Break the ampoule using forceps; ③ Pipette 0.5 mL sterile water into the lyophilized vial. After complete dissolution, spread the solution onto antibiotic plates at 200 μL per well;④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Growth conditions	37°C; 18-24h; aerobic;
Storage conditions	-80°C
Safety level	1
Sharing mode	Public welfare sharing