DH5α (containing pET22b-EGFP plasmid) E. coli|365214 |BNCC

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Escherichia coli DH5α（pET22b-EGFP）

Subculture procedure	① Prepare 1 vial of sterile water and 2 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized vial, dissolve completely, then Agar plate onto antibiotic plates (200 μL/Agar plate);④ Incubate the plates under specified conditions for 18–24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50 mL LB antibiotic-supplemented liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmid DNA from an appropriate volume of the culture as required by the experiment;
Growth conditions	37°C; 18-24h; aerobic
Storage conditions	-80°C
Safety level	1
Sharing mode	Public welfare sharing