Human coronavirus OC43（Betacoronavirus 1） RNA standard|BNCC372388 |BNCC

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Human coronavirus OC43（Betacoronavirus 1） RNA standard

Culture medium	EMEM complete medium (with 2% FBS):EMEM + 2% FBS
Subculture procedure	When using this standard, move to room temperature to be completely dissolved, shake evenly, use after instantaneous centrifugation, try to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃;3-6 days of culture time; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	1
application	The quality control is developed by Henan engineering center of industrial microbial strain and is suitable for qualitative and quantitative research and analysis of B coronavirus 1 (human coronavirus OC43), verification and evaluation of measurement methods and laboratory quality control.
Sharing mode	Public welfare sharing