Rotavirus A RNA standard|BNCC368005 |BNCC

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Rotavirus A RNA standard

Culture medium	Special medium for VR-2018: EMEM +2 μg/mL Trypsin
Subculture procedure	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	The culture temperature is 37 ℃;1-7 days of culture time; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	The strain was isolated from the diarrhoeal faeces of rotavirus-positive patients and deposited by the National Institute of Allergy and Infectious Diseases.
application	The standard is not infectious, no biosafety risk, and is suitable for qualitative and quantitative determination of rotavirus A nucleic acid by real-time fluorescence quantitative PCR and other methods, as well as laboratory quality control and kit development and evaluation.
Sharing mode	Public welfare sharing