BeNa Culture Collection
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| Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
| Subculture procedure | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
| Growth conditions | 37 ℃,24-48h, aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| application | The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated virus liquid of Shigella flexneri. It can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through digital PCR. |
| Sharing mode | Public welfare sharing |
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