BeNa Culture Collection
Culture medium | EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS |
Subculture procedure | When in use, it should be moved to room temperature (20~25 ℃) to balance for 10 minutes, shaken evenly, centrifuged briefly, and the minimum sampling amount is 200uL. After the nucleic acid extraction of the sample is completed, it is recommended to carry out the next experiment immediately, otherwise, please store it at -20±5 ℃ for use (within 24 hours). |
Growth conditions | Culture temperature 37 ℃; Culture time 3 days; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 1 |
morphology | The appearance is transparent liquid, packed in 1.5mL Frozen vials, 0.1mL per tube, totaling 5 tubes |
application | The quality control was developed byHenan engineering center of industrial microbial strain, and was obtained by adding stabilizers and digital PCR values, which can be used for method establishment, working standard assignment, method confirmation and evaluation. |
Sharing mode | Public welfare sharing |