Bovine viral diarrhea virus 1 RNA standard|BNCC364954 |BNCC

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Bovine viral diarrhea virus 1 RNA standard

Culture medium	90%DMEM-H+10%HS
Subculture procedure	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃; Culture time 2-6 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	The National Animal Disease Laboratory seals the spleens of naturally occurring BVD death cases in cattle.
application	The quality control was developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of bovine viral diarrhea virus 1, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. by adding stabilizer and digital PCR fixed value.
Sharing mode	Public welfare sharing