BeNa Culture Collection
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| Subculture procedure | Take the TSA plate and directly select the yellow suspicious colonies to inoculate L-lysine decarboxylase broth (1), L-ornithine decarboxylase broth (2), L-arginine dihydrolase (3), amino acid decarboxylase control (4), simonite citrate (5), D-sorbitol (6), L-rhamnose (7), D-sucrose (8), D-honey sugar (9), amygdalin (10), oxidase test (11). Please refer to the GB4789.40-2016 standard judgment results. (After inoculation, put 30 ℃ (Simon's citrate 37 ℃, semi-plug) and culture for 24±2h. After inoculation, sterilized paraffin should be added to cover the liquid surface of the culture medium in the amino acid decarboxylase control tube) |
| Storage conditions | 2-8 ℃ |
| Safety level | 0 |
| application | Suitable for the physiological and biochemical identification of Enterobacter sakazakii in "GB4789.40-2016 Food Microbiological Examination of Cronobacterium (Enterobacter sakazakii)" |
| Sharing mode | Public welfare sharing |