Hemorrhagic Escherichia coli O157:H7 nucleic acid detection kit (fluorescent PCR method)|BNCC360687 |BNCC

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Hemorrhagic Escherichia coli O157: H7 nucleic acid detection kit (Fluorescent PCR method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the kit is dissolved on ice, shaken and mixed evenly, nucleic acid extraction or purification reagents need to be provided by oneself, and the specific use method can be seen in the instruction manual.
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	It is suitable for the auxiliary diagnosis of EHEC O157:H7 infection in water samples of feces, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing

EHEC O157:H7 Nucleic Acid Detection Kit Certificate

1.Product information

sample name: EHEC O157:H7 Nucleic Acid Detection Kit (Fluorescence PCR Method)

Sample number: 360687

sample batch: 220302

2. Product features

Description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, is transparent liquid
Traceability	BNCC192101 enterohemorrhagic Escherichia coli O157:H7, ATCC25922 Escherichia coli
Product details	2 tubes of reaction solution, 1 tube of positive quality control material, 1 tube of negative quality control material
Field of application	It is suitable for auxiliary diagnosis of EHEC O157:H7 infection in watery stool, suspected contaminated water, food, etc.
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3.Instructions

Before use, put the kit on ice to dissolve, shake and mix well, nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. The kit should be stored at low temperature in the dark;

2. All components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid bubbles;

3. Use disposable tips, disposable gloves and wear special work clothes during the experimental operation;

4. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

5. All items in the kit should be treated as contaminants and handled in accordance with the 《General Principles of Biosafety for Microbial Biomedical Laboratories》;

5. Preservation and transportation

Long-term storage:-20 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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