Parabacteroides distasonis DNA standard|BNCC372705 |BNCC

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Parabacteroides distasonis DNA standard

Culture medium	Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0,pH:7.3±0.2(25 ℃)
Subculture procedure	① Prepare 2 pieces of the above-mentioned plates; ② After the surface of the ampoule tube is disinfected, open it in a safety cabinet, burn the top with an alcohol lamp, then quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL sterile water (placed in anaerobic environment for 24h) into a freeze-drying tube, fully dissolve the bacterial powder, and then punch 2 pieces of plates, 200 μL/piece, and coat evenly; ④ Place the plate under the above culture conditions, and the strain can be used when it grows.
Growth conditions	37 ℃; anaerobic; 48-72h
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, neat edges, opaque, white on the front, raised in the middle, smooth surface, easy to provoke, G-(red), bacilli
Separation substrate	human feces
application	Quality control of bioMérieux, Vitek and IDS products
Sharing mode	Public welfare sharing