Salmonella enterica nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC372411 |BNCC

Essential Information Related Products

Salmonella enterica nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	Pick a single colony and increase bacteria in a liquid triangular flask, then centrifuge 25mL of bacteria liquid to collect bacteria (centrifuge speed 8000r/min,30s), resuspend with 10mL PBS, and collect bacteria suspension for later use. The concentration of bacterial suspension is about 109CFU/mL, diluted to 10-6, 10-7, 10-8 gradient, take 200 μL coating count, select the number of colonies between 30 CFU ~ 300 CFU plate for counting;
Growth conditions	Culture temperature 37 ℃;18-24 hours; Aerobic atmosphere;
Storage conditions	-80 ℃
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment, this product Salmonella enteritidis nucleic acid test reagent evaluation with heat inactivated strong positive reference, for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing