BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | Before use, place the reference at room temperature for 30min, fully melt, shake and mix, extract nucleic acid and use it. The nucleic acid extraction or purification reagent shall be prepared by itself. |
Storage conditions | -80 ℃ |
Safety level | 1 |
application | This reference belongs to the heat-inactivated E. coli phage T7, by culturing E. coli host bacteria, inoculating phage T7, collecting phage. The titer and lysis efficiency of the collected phage were measured, and the count was performed using double agar. The heat-inactivated reference has no infectious pathogenicity, but retains virus particles with intact genetic material. It is mainly used forquality controls and positive controls in related nucleic acid detection processes. |
Sharing mode | Public welfare sharing |