Escherichia coli bacteriophage T1 nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC362934 |BNCC

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Escherichia coli bacteriophage T1 nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. Preparation of host bacteria liquid: Take BNCC305635(ATCC11303) Escherichia coli and culture on NA plate, grow a single colony at 37 ℃ for 18-24h, pick a single colony in a liquid triangular flask (NB medium), and culture in a shaker (37 ℃ 140r /min) until OD600 is between 0.1-0.4 (2-3h) for later use; 2. Phage culture: BNCC 259107(ATCC11303-B1) Escherichia coli phage T1 freeze-dried powder was taken, 0.5mLNB was added for dissolution, all of them were injected into the host bacteria solution between 0.1 and 0.4, shaken well, let stand for 0.5h, and then placed in a shaking table (37 ℃ 170r /min) for 24h.
Growth conditions	Culture temperature 37 ℃;18-24 hours; Aerobic;
Storage conditions	-80 ℃
Safety level	0
application	This reference product was developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It is an inactivated particle of E. coli phage T1. It can be used for the development of quality control products and standard materials of E. coli phage T1, nucleic acid detection positive control, nucleic acid detection research, etc.
Sharing mode	Public welfare sharing