BeNa Culture Collection
Subculture procedure | When using the essential control product, it should be moved to room temperature (20~25 ℃) for 10 minutes, shaken evenly, centrifuged briefly, with a minimum sampling amount of 200uL. After the sample nucleic acid extraction is completed, it is recommended to carry out the next experiment immediately, otherwise please keep it at -20±5 ℃ for later use (within 24 hours). |
Storage conditions | -80 ℃ |
Safety level | 2 |
application | The porcine reproductive and respiratory syndrome virus (PRRSV) liquid quality control product is a virus culture solution as raw material, after heat inactivation and chemical inactivation treatment, not infectious, used for porcine reproductive and respiratory syndrome virus reagent manufacturers and related testing laboratories for indoor quality control. The copy number concentration of porcine reproductive and respiratory syndrome virus gene was determined by fluorescence quantitative PCR and absolute quantitative method-digital PCR, and the reference value of quality control products was obtained, which was used for indoor quality control of porcine reproductive and respiratory syndrome virus reagent manufacturers and related laboratories for porcine reproductive and respiratory syndrome virus detection. |
Sharing mode | Public welfare sharing |
Porcine reproductive and respiratory syndrome virus nucleic acid liquid indoor quality control products
This liquid quality control product of porcine reproductive and respiratory syndrome virus is a virus culture solution as raw material, after heat inactivation and chemical inactivation treatment, it is not infectious, and is used for porcine reproductive and respiratory syndrome virus reagent manufacturers And related testing laboratories carry out indoor quality control.
1. Product information:
Sample name: Porcine reproductive and respiratory syndrome virus nucleic acid liquid indoor quality control product
Sample number: 361641
Sample batch: 220530
2.Product features:
product description | the appearance is transparent liquid, using 1.5mL frozen storage tubes, each tube is 0.5ml, a total of 5 tubes | ||
fixed value method | the copy number concentration of porcine pseudorabies virus gene was determined by fluorescence quantitative PCR and absolute quantitative method-digital PCR to obtain the standard value of reference | ||
concentration value (copies/mL) | 105~106 | reference value (copies/mL) | 1.19×105 |
store | -80℃ | validity period | 1 year |
uniformity and stability | random sampling, good uniformity of results; Long-term stability test, stability qualified | ||
scope of application | the essential control product is only used for precision testing of relevant laboratories, and can carry out quality control, measurement methods and procedures confirmation of relevant laboratories | ||
*Note: If you have any questions about quality control samples, please contact our center (BNCC) for help before use |
3. Instructions
When using the essential control product, it should be moved to room temperature (20~25℃) for 10 minutes, shake evenly, centrifuge briefly, and the minimum sampling volume is 200uL. After the nucleic acid extraction of the sample is completed, it is recommended to carry out the next experiment immediately, otherwise, please store it in - 20±5℃ Standby (within 24 hours).
4. Notes
1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.
2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.
3. The use of essential control materials should be operated by trained laboratory professionals, paying attention to biological safety protection. It should be treated as a potentially biologically infectious sample, and the operation and handling must comply with relevant regulations.
5. Preservation and transportation
Long-term storage: -80 ℃, valid for 1 year; transportation conditions: ice pack transportation, the time should not exceed 10 days.
Henan Engineering Research Center of Industrial Microbiology
website: www.bncc .org .cn tel: 400-6699-833