BeNa Culture Collection
Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, yellow on the back, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G + (blue-purple), cocci, purity: pure |
Sharing mode | Public welfare sharing |