Saccharomyces cerevisiae quantitative strain|BNCC361907 |BNCC

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Saccharomyces cerevisiae Meyen ex E.C. Hansen

Culture medium	YM medium (English name: YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	30 ℃;24-48h; Aerobic;
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, milky white on the front, milky white on the back, convex in the middle, smooth surface, moist texture, bright surface, easy to stir up, G + (blue-purple), cocci, purity: pure
Sharing mode	Public welfare sharing