BeNa Culture Collection
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BeNa Culture Collection
| Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculture procedure | 1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After full dissolution, it is (2.5±1.0)× 10 6cfu/ml, which is absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it; |
| Growth conditions | 37 ℃; Aerobic; |
| Growth characteristics | Gram-positive bacteria, spherical, chain-like arrangement, fermenting glucose to produce lactic acid. |
| Storage conditions | -80 ℃ |
| morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth and bright surface, moist texture, easy to pick up, G + (blue-purple), cocci, purity: pure |
| Separation substrate | blood - human |
| application | Type strain, fermentation of glucose to produce lactic acid |
| Sharing mode | Public welfare sharing |
Streptococcus salivarius
Strain number: BNCC 354947
Name: Streptococcus salivarius
Product format: quantitative bacteria tablets Number of viable bacteria: (2.5±1.0)×10^6CFU/bottle
Product batch: 21122001 Valid until: 2022.12.19
Inspection date: 2021.12.21 Sampling ratio: ≥2%
Report number:RS21354947-1 Biosafety level: 1,handle in ultra-clear table or safety cabinet
| Item | Inspection description | results |
| stain microscopy | gram negative and positive, cell morphology observed under microscope | G + cocci, spherical, chain-like arrangement |
| colony morphology | observation of single colony morphology | pale yellow, raised, moist, bright |
| Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
| Reference value of live bacteria | Non-selective medium plate, viable count | 2.0×10^6CFU/bottle |
| Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
| test conclusion | After the BNCC quality control inspection system quality inspection, the product is qualified | |
Product list: One bottle of (2.5±1.0)×10^6CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;
Validity period: unopened, below -20 ℃, 12 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. After full dissolution, it is (2.5±1.0)×10^6CFU/bottle, according to the test requirements according to the proportion of suction can be;
4. Please use up immediately after opening, and do not store it;
Notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
② Used bacteria-carrying consumables, please autoclave and discard them.
