Slow-growing rhizobium for cowpea|335824 |BNCC

Essential Information Related Products

Bradyrhizobium sp.

Growth conditions	28-30°C, aerobic
Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve thoroughly, then transfer back to the liquid test tube and mix well; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, pink on top, centrally convex, smooth surface, glossy appearance, viscous texture, moist consistency, G- (red), bacillus, purity: pure
Sharing mode	Public welfare sharing