BeNa Culture Collection
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| Culture medium | BNCC352238 |
| Description | MRS agar medium |
| Composition | Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37 ℃; Anaerobic; 24-48 hours |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ refrigerated |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, pink on the front, raised in the middle, smooth and bright on the surface, sticky and moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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