Salmonella typhimurium|351962 |BNCC

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Salmonella typhimurium

Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	37°C; 18-24h; aerobic;
Storage conditions	-20℃
Safety level	2
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, pink on top, centrally convex, smooth surface, glossy appearance, viscous texture, moist consistency, G- (red), bacillus, purity: pure
Sharing mode	Public welfare sharing

Salmonella typhimurium

No. : 351962

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 2

Validity period: 5 years

Use : pharmacopoeia, national standard and other control quality control strains

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6)If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And the strains can be used when they grow

Growth conditions temperature: 37 ℃ Gas environment: aerobic Time: 18-24h

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