BeNa Culture Collection
info@bncc.com
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
| Culture medium | 2216E seawater agar (2216E): peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, agar 15.0g, distilled water 1000ml,pH 7.4-7.8. Sterilization at 121 ℃ for 15min. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 30 ℃; Aerobic |
| Sharing mode | Public welfare sharing |
Quality control sample of total bacterial count in drinking water
BNCC374001
Biochemical ldentification Kit for Klebsiella pneumoniae
BNCC361613
Water quality fecal coliform standard quality control sample (Membrane membrane method)
BNCC360398
Quantitative strains of pseudomonas aeruginosa
BNCC353739
Pseudomonas aeruginosa quality control sample
BNCC370599
Quantitative strains of candida albicans
BNCC353801
- English
- Japanese