Oceanisphaera ostreae T-w6(T) |BNCC356276 |BNCC

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Oceanisphaera ostreae T-w6(T) -BNCC
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  • Oceanisphaera ostreae T-w6(T) -BNCC

Oceanisphaera ostreae T-w6(T) 

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  • number：BNCC356276
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
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Oceanisphaera ostreae T-w6(T) 
Culture medium 2216E seawater agar (2216E): peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, agar 15.0g, distilled water 1000ml,pH 7.4-7.8. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃; Aerobic
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