BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Growth conditions | 30 ℃;18-24h; Aerobic; |
Storage conditions | 2-8 ℃ |
morphology | Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Off-white Protuberance: Middle convex surface: Smooth and bright Texture: Wet and sticky |
Sharing mode | Public welfare sharing |
Cupriavidus sp.
Storage conditions: 2~8 ℃
No. 356124
Product format: 90mm flat plate
Validity period: 10 days
Biosafety level: 1, handle in ultra-clear table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions: 30℃, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.0. Sterilize at 121°C for 15min.
Recovery steps:
In a sterile environment, use a sterile inoculation ring to pick up a sufficient amount of plate colonies, transfer them to a plate or liquid culture medium, and culture them under suitable conditions for 18-24 hours. the bacteria can be used when they grow out.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
Item | test results |
viability | good viability,in 24 hours,liquid medium become turbid, colony is typical on marked plate |
colony morphology: (above) |
Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle raised surface: smooth and bright texture: moist and viscous |
Conclusion | good viability, no abnormal colony morphology, qualified |