Bacillus subtilis|BNCC353754 |BNCC

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Bacillus subtilis

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Growth conditions	37 ℃, aerobic
Growth characteristics	Gram staining is positive, rod-shaped, rarely strands, uniform staining; flagella are lateral and movable; endospores are 0.8 × 1.5-1.8 μm, and the surface of free spores is poorly colored; the colonies on the agar culture medium are round or Irregular shape; the surface color is dark, opaque, can wrinkle, can change to cream or brown; the shape of the colony varies greatly with the composition of the culture medium; strictly nourishing or facultative anaerobic.
Storage conditions	-80 ℃
application	Research; Analytical testing. Antibiotic titer determination, temporarily used for the verification of midecamycin. Antibody analysis, positive control strain of GB 4789.2-2010 colony count determination, standard strain of GB 4789.28 medium and reagent quality requirements, quality control strain of Chinese pharmacopoeia, 2015 edition. Provide quality inspection report.
Sharing mode	Public welfare sharing

Staphylococcus aureus

No. : 353754

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 1

Validity period: 5 years

Use : pharmacopoeia, national standard and other control quality control strains

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6)If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And the strains can be used when they grow

Growth conditions temperature: 37 ℃ Gas environment: aerobic Time: 18-24h

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