BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
| Culture medium | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 37°C;48-72h; Anaerobic |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
Standard quality control samples of Escherichia coli in drinking water (multi-tube fermentation method)
BNCC360415
Biochemical ldentification Kit for salmonella
BNCC362198
Water quality fecal coliform standard quality control sample (Membrane membrane method)
BNCC360398
Quantitative strains of Staphylococcus aureus
BNCC381462
Reference material of Aerobic plate Count
BNCC372444
Lead in Whole Blood
BNCC372157
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