Absorbent Streptomyces subspecies|BNCC337576 |BNCC

Essential Information Certificate Related Products

Streptomyces hygroscopicus subsp. hygroscopicus

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Filamentous fungi, the colonies are obvious on Streptomyces medium No. 2, the hyphae are white, dense and low, and the hyphae are vigorous and spread.
Separation substrate	Easter island soil
application	Rapamycin production
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces hygroscopicus subsp. hygroscopicus

2. BNCC No.:337576

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. ISP-2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH7.2±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3, Temperature: 26 ℃

4. precautions

1. Routine culture time: bacteria 24-48h, yeast 72h, mold 5-7d, macrofungi 7-10d.

2. Please transfer the inclined plane of the test tube as soon as possible. the storage time shall not exceed 3 months.

3. For the first time, please activate according to the recommended conditions in this manual. BNCC is not responsible for the loss of inactivation of strains caused by other types of culture media or culture conditions.

4. Bacteria-carrying waste should be discarded after autoclaving.

Download certificate