Streptococcus dysgalactiae subsp. dysgalactiae|BNCC337480 |BNCC

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Streptococcus dysgalactiae subsp. dysgalactiae

Culture medium	Columbia Blood Plate (CA-B): Casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat and stomach enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3 0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilizing the surface of the plate, open it in the cabinet; (3) suck 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, transfer it into a fresh plate, and spread it evenly; (5) Place the plate under the above culture conditions and culture
Growth conditions	37 ℃;18-24h;5% CO2
Storage conditions	2-8 ℃
morphology	Size: smaller color: off-white shape: round edge: neat edge dry and wet: wet and smooth: smooth transparency: opaque uplift: convex
Separation substrate	Infected cow breasts
Sharing mode	Public welfare sharing

Streptococcus dysgalactiae subsp.

Storage conditions : 2~8 ℃

No. : 337480

Product format: freeze dried, 200ul, or flat plate 1

Validity period : freeze-dried tube for 24 months, flat plate for 7 days

Biosafety level : 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Agar plates can be used directly. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37 ℃,5% CO₂, Columbia blood plate (finished product)

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 20h, Colombian blood strain layer is obvious
colony morphology	size: smaller color: off-white shape: round edge: neat edge dry and wet: moist and smooth: smooth transparency: opaque uplift: bulge
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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