BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. |
| Subculture procedure | (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions. |
| Growth conditions | 28 ℃;5-7 days; Aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi, with obvious colonies, light yellow, green spores and yellow back of the medium on the integrated PDA medium. |
| Model strain | no |
| application | High concentration copper sulfate resistance |
| Sharing mode | Public welfare sharing |
Penicillium ochrochloron Biooourge
Storage conditions : 2~8 ℃
No. : 336589
Product Format : freeze dried, 200ul
Validity period: 6 years
Biosafety level : 1,handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.
Growth Conditions :28 ℃, aerobic, comprehensive PDA,5-7 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery steps:
(1)Prepare 1-2 of above mentioned plates;
(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test results |
| viability | good viability, in 5-7 days strain layer is obvious |
| colony morphology: (above) | small filamentous fungi have obvious colonies, dense and vigorous hyphae on comprehensive PDA medium, producing cyan spores. |
| conclusion | good viability, no abnormal colony morphology, qualified |
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