Aspergillus oryzae (Ahlburg) Cohn|BNCC336577 |BNCC

Aspergillus oryzae (Ahlburg) Cohn

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number：BNCC336577
Form：
Small filamentous fungi, with obvious colonies on malt extract medium, white hyphae, yellow-green spores in the later period, raised in the center of the colony and no spores, and brown on the back of the medium.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Aspergillus oryzae (Ahlburg) Cohn

Culture medium	Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on malt extract medium, white hyphae, yellow-green spores in the later period, raised in the center of the colony and no spores, and brown on the back of the medium.
application	Brewing soy sauce.
Sharing mode	Public welfare sharing

1.Description

1. Name: Aspergillus oryzae (Ahlburg) Cohn

2. BNCC No.:336577

3. Biosafety level : 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, 5 Be′ Wort agar: 5 Be Wort 1.0L, natural pH.

2. Atmosphere:aerobic

3, Temperature: 28-30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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