Aspergillus ficuum|BNCC225981 |BNCC

Aspergillus ficuum

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number：BNCC225981
Form：
Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the plate layer hyphae is white in the early stage, spreading and growing, and in the late stage Yellow-green spores are produced, and the back of the medium is khaki.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Aspergillus ficuum

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions.
Growth conditions	28 ℃,3-5 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the plate layer hyphae is white in the early stage, spreading and growing, and in the late stage Yellow-green spores are produced, and the back of the medium is khaki.
Sharing mode	Public welfare sharing

Aspergillus ficuum

BNCC No.：225981

Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA agar: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 ±0.2.

2. Atmosphere：aerobic

3. Temperature：25-28 ℃,4 days

4.Notes：

1.Normal culturing time：24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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