Laceyella sacchari|BNCC186410 |BNCC

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Laceyella sacchari-BNCC
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  • Laceyella sacchari-BNCC

Laceyella sacchari

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  • number：BNCC186410
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
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Laceyella sacchari
Culture medium Oat flour agar -1 (English name: ISP-3): oat flour 20.0g, trace salt solution 1.0ml, agar 15.0g, distilled water 1.0L,pH7.2,121 ℃,15min sterilization. [Note] Formula of trace salt solution: FeSO4 · 7H2O 0.1g,MnCl2 · 4H2O 0.1g,ZnSO4 · 7H2O 0.1g, distilled water 100.0ml.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 55 ℃;48-72h; Aerobic
Storage conditions 2-8 ℃
Separation substrate Bagasse
Sharing mode Public welfare sharing
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