Bacillus coagulans|BNCC168437 |BNCC

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Bacillus coagulans

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front and back, flat in shape, smooth in surface, bright in surface, moist in texture, easy to stir up, G + (blue-purple), bacilli, purity: pure
Separation substrate	Dairy products
application	Glycoside hydrolase inhibitor, L(+) lactic acid
Sharing mode	Public welfare sharing

Bacillus coagulans

Storage conditions : 2~8 ℃

No. : 168437

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37 ℃, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare a test tube containing 5~10mL of liquid culture medium and 2 plates;

②Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ suck 0.5mL of liquid culture medium into freeze-dried tube, fully dissolve and return to liquid test tube again, and mix well;

④ Absorb 0.2mL of bacterial suspension into the plate, coat evenly, and repeat twice to obtain two plates;

⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 18-24hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Untidy Transparency: Opaque Color: Off-white Bulge: Flat Surface: Bright and smooth Texture: Moist and sticky
Conclusion:	good viability, no abnormal colony morphology, qualified

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