BeNa Culture Collection
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| Culture medium | BNCC360798 |
| Description | Colombia Agar Chocolate Plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 7-10 days; anaerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above (pre-incubate in anaerobic environment for 24h to remove oxygen); ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush the contents; ③ Pipette 0.5mL sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) into the freeze-dried vial. After fully dissolving the powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The strain is ready for use once colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 0.5–1 mm; circular, with regular margins; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; Gram-positive (blue-violet); bacilli; Purity: Pure |
| Sharing mode | Public welfare sharing |
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