Bacillus genus|385162 |BNCC

Essential Information Related Products

Bacillus sp.

Culture medium	BNCC360798
Description	Colombia Agar Chocolate Plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃)
Growth conditions	37°C; 7-10 days; anaerobic;
Subculture procedure	① Prepare 1-2 plates as above (pre-incubate in anaerobic environment for 24h to remove oxygen); ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush the contents; ③ Pipette 0.5mL sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) into the freeze-dried vial. After fully dissolving the powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The strain is ready for use once colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular, with regular margins; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; Gram-positive (blue-violet); bacilli; Purity: Pure
Sharing mode	Public welfare sharing