BeNa Culture Collection
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| Culture medium | BNCC360847 |
| Description | Cooked Meat Medium |
| Composition | Peptone:30.0g, Beef Extract Powder:3.0g, Yeast Extract Powder:5.0g, Soluble Starch:2.0g, Glucose:3.0g, NaH2PO4:5.0g, pH:7.0-7.4(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic; |
| Subculture procedure | ① Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into one test tube (the culture medium needs to be deoxygenated 24 hours in advance); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Turbidity of bacterial liquid |
| Sharing mode | Public welfare sharing |
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